{"id":53613,"date":"2024-03-22T10:09:24","date_gmt":"2024-03-22T09:09:24","guid":{"rendered":"https:\/\/biotech.uwr.edu.pl\/?page_id=53613"},"modified":"2025-11-24T13:58:30","modified_gmt":"2025-11-24T12:58:30","slug":"advanced-imaging-and-cytometry-laboratory","status":"publish","type":"page","link":"https:\/\/biotech.uwr.edu.pl\/en\/advanced-imaging-and-cytometry-laboratory\/","title":{"rendered":"Advanced Imaging and Cytometry Laboratory"},"content":{"rendered":"\n<div class=\"wp-block-media-text alignfull is-stacked-on-mobile is-vertically-aligned-bottom is-image-fill-element bs-naglowek-szablonu-kolumny\" style=\"grid-template-columns:40% auto\"><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"746\" height=\"1024\" src=\"https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/idub_logo_rgb-1-746x1024.jpg\" alt=\"logo idub\" class=\"wp-image-53481 size-large\" style=\"object-position:60% 98%\" srcset=\"https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/idub_logo_rgb-1-746x1024.jpg 746w, https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/idub_logo_rgb-1-218x300.jpg 218w, https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/idub_logo_rgb-1-768x1055.jpg 768w, https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/idub_logo_rgb-1-17x24.jpg 17w, https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/idub_logo_rgb-1-26x36.jpg 26w, https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/idub_logo_rgb-1-35x48.jpg 35w, https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/idub_logo_rgb-1.jpg 1004w\" sizes=\"auto, (max-width: 746px) 100vw, 746px\" \/><\/figure><div class=\"wp-block-media-text__content\">\n<div class=\"wp-block-bonasoft-modular-breadcrumbs-block\"><div class=\"bs_add_breadcrumb_trail\"><\/div><\/div>\n\n\n\n<hr class=\"wp-block-separator has-text-color has-black-color has-css-opacity has-black-background-color has-background is-style-wide bs-border-kontakt\"\/>\n\n\n\n<h1 class=\"wp-block-heading bs-naglowek-podstrony\">Advanced Imaging and Cytometry Laboratory<\/h1>\n\n\n\n<p class=\"has-text-align-center\"><em>The purchase of the equipment was financially supported by the Research Equipment Fund (FAB) of the &#8220;Excellence Initiative \u2014 Research University&#8221; (IDUB) program for the University of Wroclaw.<\/em><\/p>\n\n\n\n<p>Key personnel:<br><a href=\"https:\/\/old.uni.wroc.pl\/en\/search-for-employees\/employee\/?p_id=590172\" target=\"_blank\" rel=\"noreferrer noopener\">dr Micha\u0142 Majkowski<\/a><\/p>\n\n\n\n<p>Contact: michal.majkowski@uwr.edu.pl<\/p>\n\n\n\n<p><a href=\"https:\/\/orcid.org\/0000-0001-9252-5784\" target=\"_blank\" rel=\"noreferrer noopener\"> <\/a><\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-ugb-container ugb-container bs-strona-konferencje ugb-0acb16f ugb-container--v2 ugb-container--design-plain ugb-main-block\"><style>@media screen and (min-width:768px){.ugb-0acb16f.ugb-container{margin-bottom:0% !important;margin-right:6% !important;margin-left:6% !important}}@media screen and (min-width:768px){.ugb-0acb16f > .ugb-inner-block > .ugb-block-content > *{padding-top:0px !important;padding-bottom:0px !important;padding-right:0px !important;padding-left:0px !important}}<\/style><div class=\"ugb-inner-block\"><div class=\"ugb-block-content\"><div class=\"ugb-container__wrapper ugb-0acb16f-wrapper\"><div class=\"ugb-container__side\"><div class=\"ugb-container__content-wrapper ugb-0acb16f-content-wrapper\">\n<div class=\"wp-block-ugb-accordion ugb-accordion ugb-1dbaa19 ugb-accordion--v2 ugb-accordion--design-plain ugb-accordion--single-open ugb-main-block\" aria-expanded=\"false\"><style>.ugb-1dbaa19 .ugb-accordion__title{line-height:1em !important}.ugb-1dbaa19 .ugb-accordion__arrow{fill:#ffffff}.ugb-1dbaa19 .ugb-accordion__item{border:none !important}@media screen and (min-width:768px){.ugb-1dbaa19.ugb-accordion{margin-top:0px !important;margin-bottom:0px !important;margin-right:0px !important;margin-left:0px !important;padding-top:0px !important;padding-right:0px !important;padding-bottom:0px !important;padding-left:0px !important}}<\/style><div class=\"ugb-inner-block\"><div class=\"ugb-block-content\"><div class=\"ugb-accordion__item\"><div class=\"ugb-accordion__heading\" role=\"button\" tabindex=\"0\"><h3 class=\"ugb-accordion__title\"><strong>Equipment<\/strong> <strong>and Service<\/strong><\/h3><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 20 20\" class=\"ugb-accordion__arrow\" width=\"20\" height=\"20\"><path d=\"M16.7 3.3L10 10 3.3 3.4 0 6.7l10 10v-.1l10-9.9z\"><\/path><\/svg><\/div><div class=\"ugb-accordion__content\" role=\"region\"><div class=\"ugb-accordion__content-inner\">\n<div class=\"wp-block-ugb-accordion ugb-accordion ugb-35cdda6 ugb-accordion--v2 ugb-accordion--design-basic ugb-main-block\" aria-expanded=\"false\"><div class=\"ugb-inner-block\"><div class=\"ugb-block-content\"><div class=\"ugb-accordion__item\"><div class=\"ugb-accordion__heading\" role=\"button\" tabindex=\"0\"><h4 class=\"ugb-accordion__title\">Confocal microscope Stellaris 8 with FLIM and STED modules<\/h4><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 20 20\" class=\"ugb-accordion__arrow\" width=\"20\" height=\"20\"><path d=\"M16.7 3.3L10 10 3.3 3.4 0 6.7l10 10v-.1l10-9.9z\"><\/path><\/svg><\/div><div class=\"ugb-accordion__content\" role=\"region\"><div class=\"ugb-accordion__content-inner\">\n<p>Research confocal microscope. FLIM is an imaging technique in which each pixel contain information not only about fluorescence signal intensity but also about fluorescence lifetime. Measuring the latter might provide information about molecular environment of the probe. This technique is also useful in FRET measurement. STED uses 660 nm depletion laser line and is combined with fluorescence lifetime measurement. This allow &#8211; in theory &#8211; to achieve resolution in XY in the region of 70-80 nm. The system also offers FCS measurements.<\/p>\n\n\n\n<p>Dry, water- and oil-immersion optics, incubation chamber with CO2. Wide range of excitation lines and great flexibility in detection range.<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-ugb-accordion ugb-accordion ugb-9e91e80 ugb-accordion--v2 ugb-accordion--design-basic ugb-main-block\" aria-expanded=\"false\"><div class=\"ugb-inner-block\"><div class=\"ugb-block-content\"><div class=\"ugb-accordion__item\"><div class=\"ugb-accordion__heading\" role=\"button\" tabindex=\"0\"><h4 class=\"ugb-accordion__title\">Elyre 7 microscope<\/h4><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 20 20\" class=\"ugb-accordion__arrow\" width=\"20\" height=\"20\"><path d=\"M16.7 3.3L10 10 3.3 3.4 0 6.7l10 10v-.1l10-9.9z\"><\/path><\/svg><\/div><div class=\"ugb-accordion__content\" role=\"region\"><div class=\"ugb-accordion__content-inner\">\n<p>Research super-resolution microscope. Uses standard samples stained with the most popular fluorophores and employs structured pattern of illumination to achieve resolution in the region of 70 nm. Also offers TIRF, STORM and PALM imaging. Dry, water- and oil-immersion optics, incubation chamber, 405 nm, 488 nm, 561nm, 633 nm laser lines. Two cameras: sCMOS and back-thinned EMCCD. Resolution in Z in the region of 300 nm. Fluorescence filters for four typical fluorofores: DAPI (or similar), Cy2 (or similar), Cy3 (or similar) and Cy5 (or similar).<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-ugb-accordion ugb-accordion ugb-b1e12c4 ugb-accordion--v2 ugb-accordion--design-basic ugb-main-block\" aria-expanded=\"false\"><div class=\"ugb-inner-block\"><div class=\"ugb-block-content\"><div class=\"ugb-accordion__item\"><div class=\"ugb-accordion__heading\" role=\"button\" tabindex=\"0\"><h4 class=\"ugb-accordion__title\">Opera Phenix HTS\/HCS microscope<\/h4><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 20 20\" class=\"ugb-accordion__arrow\" width=\"20\" height=\"20\"><path d=\"M16.7 3.3L10 10 3.3 3.4 0 6.7l10 10v-.1l10-9.9z\"><\/path><\/svg><\/div><div class=\"ugb-accordion__content\" role=\"region\"><div class=\"ugb-accordion__content-inner\">\n<p>Spinning disc based confocal microscope, optimized for large scale imaging, is able to produce vast number of images in relatively short time. Equipped with robotic arm that shuttles samples between incubator\/plate rack and microscope. An injector allows to add a compound of interest to the sample while capturing images what allows to image very fast processes. Harmony software does smooth image processing and quantification. The system also allows high scale FRET imaging between CFP and YFP.<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-ugb-accordion ugb-accordion ugb-0be8e58 ugb-accordion--v2 ugb-accordion--design-basic ugb-main-block\" aria-expanded=\"false\"><div class=\"ugb-inner-block\"><div class=\"ugb-block-content\"><div class=\"ugb-accordion__item\"><div class=\"ugb-accordion__heading\" role=\"button\" tabindex=\"0\"><h4 class=\"ugb-accordion__title\">Service<\/h4><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 20 20\" class=\"ugb-accordion__arrow\" width=\"20\" height=\"20\"><path d=\"M16.7 3.3L10 10 3.3 3.4 0 6.7l10 10v-.1l10-9.9z\"><\/path><\/svg><\/div><div class=\"ugb-accordion__content\" role=\"region\"><div class=\"ugb-accordion__content-inner\">\n<p>Consultation on experimental desing;<br>Training;<br>Cells sorting and analysis by flow cytometry;<br>Image quantification and analysis.<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"576\" src=\"https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/nieformalna-1024x576.png\" alt=\".\" class=\"wp-image-53628\" srcset=\"https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/nieformalna-1024x576.png 1024w, https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/nieformalna-300x169.png 300w, https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/nieformalna-768x432.png 768w, https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/nieformalna-24x14.png 24w, https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/nieformalna-36x20.png 36w, https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/nieformalna-48x27.png 48w, https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/nieformalna.png 1280w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\">Image courtesy of Magdalena Kot, Aleksandra Simiczyjew, Micha\u0142 Majkowski.<\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Research confocal microscope. FLIM is an imaging technique in which each pixel contain information not only about fluorescence signal intensity but also about fluorescence lifetime. Measuring the latter might provide information about molecular environment of the probe. This technique is also useful in FRET measurement. STED uses 660 nm depletion laser line and is combined [&hellip;]<\/p>\n","protected":false},"author":1299,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[],"tags":[],"class_list":["post-53613","page","type-page","status-publish","hentry"],"featured_image_urls_v2":{"full":"","thumbnail":"","medium":"","medium_large":"","large":"","1536x1536":"","2048x2048":"","menu-24x24":"","menu-36x36":"","menu-48x48":""},"post_excerpt_stackable_v2":"<p>Advanced Imaging and Cytometry Laboratory The purchase of the equipment was financially supported by the Research Equipment Fund (FAB) of the &#8220;Excellence Initiative \u2014 Research University&#8221; (IDUB) program for the University of Wroclaw. Key personnel:dr Micha\u0142 Majkowski Contact: michal.majkowski@uwr.edu.pl Equipment and Service Confocal microscope Stellaris 8 with FLIM and STED modules Research confocal microscope. FLIM is an imaging technique in which each pixel contain information not only about fluorescence signal intensity but also about fluorescence lifetime. Measuring the latter might provide information about molecular environment of the probe. This technique is also useful in FRET measurement. STED uses 660 nm&hellip;<\/p>\n","category_list_v2":"Uncategorized","author_info_v2":{"name":"mskuza","url":"https:\/\/biotech.uwr.edu.pl\/en\/author\/mskuza\/"},"comments_num_v2":"0 comments","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Advanced Imaging and Cytometry Laboratory - Wydzia\u0142 Biotechnologii<\/title>\n<meta name=\"description\" content=\"Wydzia\u0142 Biotechnologii\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/biotech.uwr.edu.pl\/en\/advanced-imaging-and-cytometry-laboratory\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Advanced Imaging and Cytometry Laboratory - Wydzia\u0142 Biotechnologii\" \/>\n<meta property=\"og:description\" content=\"Wydzia\u0142 Biotechnologii\" \/>\n<meta property=\"og:url\" content=\"https:\/\/biotech.uwr.edu.pl\/en\/advanced-imaging-and-cytometry-laboratory\/\" \/>\n<meta property=\"og:site_name\" content=\"Wydzia\u0142 Biotechnologii\" \/>\n<meta property=\"article:modified_time\" content=\"2025-11-24T12:58:30+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/biotech.uwr.edu.pl\/wp-content\/uploads\/sites\/261\/2024\/03\/idub_logo_rgb-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1004\" \/>\n\t<meta property=\"og:image:height\" content=\"1379\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/biotech.uwr.edu.pl\/en\/advanced-imaging-and-cytometry-laboratory\/\",\"url\":\"https:\/\/biotech.uwr.edu.pl\/en\/advanced-imaging-and-cytometry-laboratory\/\",\"name\":\"Advanced Imaging and Cytometry Laboratory - 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