PhD Students

• Aleksandra Chorążewska : room 3.09., aleksandra.chorazewska@uwr.edu.pl
• Krzysztof Ciura : room 3.09., krzysztof.ciura@uwr.edu.pl
• Paulina Gregorczyk : room 3.09., paulina.gregorczyk@uwr.edu.pl
• Julia Ptak : room 3.09., julia.ptak2@uwr.edu.pl
• Subhradeep Haldar : room 3.29., subhradeep.haldar@uwr.edu.pl

RESEARCH TOPICS

• Comparison of intracellular mechanism of calcitriol activity (hormonally active form of vitamin D3) with mechanism of an activity of its semi-selective analogues
• Examination of regulation mechanism of differentiation of the cells by nuclear receptor for vitamin D
• Designing of human Fibroblast Growth Factor-1 (FGF-1) variants resistant to proteolysis
• Examination of phosphorylation mechanism of proteins

Selected Publications (2020-2024):

2024

• Chorążewska A, Regan D, Kalka M, Ciura K, Porębska N, Opaliński Ł, Thermostable phenylacetic acid degradation protein TtPaaI from Thermus thermophilus as a scaffold for tetravalent display of proteins, Protein Expression and Purification, 2024.
• Kalka M, Chorążewska A, Gędaj A, Żukowska D, Ciura K, Biaduń M, Gregorczyk P, Ptak J, Porębska N, Opaliński Ł, Engineered intrinsically fluorescent galectin-8 variants with altered valency, ligand recognition and biological activity, International Journal of Biological Macromolecules, 2024.
• Biadun M, Karelus R, Krowarsch D, Opaliński Ł, Zakrzewska M, FGF12: biology and function, Differentiation, 2024.
• Biadun M, Sochacka M, Kalka M, Chorążewska A, Karelus R, Krowarsch D, Opaliński Ł, Zakrzewska M, Uncovering key steps in FGF12 cellular release reveals a common mechanism for unconventional FGF protein secretion, Cellular and Molecular Life Sciences, 2024.
• Gędaj A, Gregorczyk P, Żukowska D, Chorążewska A, Ciura K, Kalka M, Porębska N, Opaliński Ł, Glysocylation of FGF/FGFR: an underrated sweet code regulating cellular signaling programs. Cytokines and Growth Factor Reviews, 2024.
• Żukowska D, Chorążewska A, Ciura K, Gędaj A, Kalka M, Poźniak M, Porębska N, Opaliński Ł, The diverse dependence of galectin-1 and -8 on multivalency for the modulation of FGFR1 endocytosis, Cell Communication and Signaling, 2024.
• Gędaj A, Chorążewska A, Ciura K, Karelus R, Żukowska D, Biaduń M, Kalka M, Zakrzewska M, Porębska N, Opaliński Ł, The intracellular interplay between galectin-1 and FGF12 in the assembly of ribosome biogenesis complex. Cell Communication and Signaling, 2024.
• Grown G, Marchwicka A, Marcinkowska E, Vitamin D and immune system, Advanced Food and Nutrition Research, 2024.
• Krzyscik M, Porębska N, Opaliński Ł, Otlewski J, Targeting HER2 and FGFR-positive cancer cells with a bispecific cytotoxic conjugate combining anti-HER2 Affibody and FGF2, International Journal of Biological Macromolecules, 2024.

2023

• Gebert M, Bartoszewska S, Opaliński Ł, Collawn JF, Bartoszewski R, IRE1-mediated degradation of pre-miR-301a promotes apoptosis through upregulation of GADD45A, Cell Communication and Signaling, 2023.
• Porębska N, Ciura K, Chorążewska A, Zakrzewska M, Otlewski J, Opaliński Ł, Protein-based strategies for generation of multivalent protein-drug conjugates-towards upgraded precision and efficiency of drug delivery into cancer cells, Biotechnology Advances, 2023.
• Żukowska D, Gędaj A, Porębska N, Poźniak M, Czyrek A, Krzyścik M, Krowarsch D, Otlewski J, Zakrzewska M, Opaliński Ł, Receptor clustering by a pecise set of extracellular galectins initiates FGFR signaling, Cellular and Molecular Life Sciences, 2023.
• Marchwicka A, Nowak K, Satyr A, Wołowiec D, Marcinkowska E, Immuno-Stimulating Activity of 1,25-Dihydroxyvitamin D in Blood Cells from Five Healthy People and in Blasts from Five Patients with Leukemias and Pre-Leukemic States, International Journal of Molecular Sciences, 2023.
• Gędaj A, Żukowska D, Porębska N, Poźniak M, Czyrek A, Krzyścik M, Krowarsch D, Otlewski J, Zakrzewska M, Opaliński Ł, Gaelctins use N-glycans of FGFs to capture growth factors in the extracellular matrix and fine-tune their signaling, Cell Communication and Signaling, 2023.
• Gregorczyk P, Porębska N, Żukowska D, Chorążewska A, Gędaj A, Malinowska A, Otlewski J, Zakrzewska M, Opalinski Ł, N-glycosylation acts as a switch for FGFR1 trafficking between the plasma membrane and nuclear envelope, Cell Communication and Signaling, 2023.
• Biadun M, Sochacka M, Karelus R, Baran K, Czyrek A, Otlewski J, Krowarsch D, Opaliński Ł, Zakrzewska M, FGF homologous factors are secreted from cells to induce FGFR-mediated anti-apoptotic response, FASEB Journal, 2023.

2022

• Marchwicka A, Nowak U, Grembowska A, Jakuszak A, Poręba P, Marcinkowska E, Overexpressed fibroblast growth factor receptors increase 1,25-dihydroxyvitamin D-dependent differentiation of acute myeloid leukemia cells, Journal of Steroid Biochemistry and Molecular Biology, 2022.
• Marcinkowska E, Vitamin D Derivatives in Acute Myeloid Leukemia: The Matter of Selecting the Right Targets, Nutrients, 2022.
• Marcinkowska E, Brown G, Editorial: Vitamin D and COVID-19: New Mechanistic and Therapeutic Insights, Frontiers in Pharmacology, 2022.
• Krzyścik M, Opaliński Ł, Otlewski J, Cyclic and dimeric fibroblast growth factor 2 variants with high biomedical potential, International Journal of Biological Macromolecules, 2022.
• Pozniak M, Zukowska D, Gedaj A, Krzyscik M, Porebska N, Zakrzewska M, Otlewski J and Opaliński Ł, The significance of cell surface N-glycosylation for internalization and potency of cytotoxic conjugates targeting receptor tyrosine kinases, International Journal of Molecular Sciences, 2022.
• Sochacka M, Karelus R, Biaduń M, Opaliński Ł, Krowarsch D, Otlewski J, Zakrzewska M, FGF12 is a novel component of the nucleolar NOLC1/TCOF1 ribosome biogenesis complex, Cell Communication and Signaling, 2022.
• Szymczyk J, Sochacka M, Chudy P, Opaliński Ł, Otlewski J, Zakrzewska M, FGF1 protects FGFR1-overexpressing cancer cells against taltobulin by activating AKT via two independent mechanisms, Frontiers in Oncology, 2022.

2021

• Poźniak M, Porębska N, Krzyścik MA, Sokołowska-Wędzina A, Jastrzębski K, Zakrzewska M, Otlewski J and Opaliński Ł. The cytotoxic conjugate of highly internalizing engineered tetravalent antibody T-Fc for targeting FGFR1-overproducing cancer cells, Molecular Medicine, 2021.
• Poźniak M, Porębska N, Jastrzębski K, Krzyścik M, Kucińska M, Zarzycka W, Barbach A, Zakrzewska M, Otlewski J, Miączyńska M, Opaliński Ł, Modular self-assembly system for development of oligomeric, highly internalizing and potent cytotoxic conjugates targeting fibroblast growth factor receptors, Journal of Biomedical Science, 2021.
• Porębska N, Knapik A, Poźniak M, Krzyścik M, Zakrzewska M, Otlewski J, Opaliński Ł, Intrinsically fluorescent oligomeric cytotoxic conjugate toxic for FGFR1 overproducing cancers, Biomacromolecules, 2021.
• Pozniak M, Zarzycka W, Porebska N, Kanpik A, Marczakiewicz-Perera P, Małgorzata Zakrzewska M, Otlewski J, Opaliński Ł, FGF1 fusions with the Fc fragment of IgG1 for the assembly of GFPpolygons mediated multivalent complexes targeting FGFRs, Biomolecules, 2021.
• Porebska N, Pozniak M, Krzyscik MA, Knapik A, Czyrek A, Kucinska M, Jastrzebski K, Zakrzewska M, Otlewski J and Opaliński Ł, Dissecting biological activities of fibroblast growth factor receptors by coiled-coil-mediated oligomerization of FGF1, International Journal of Biological Macromolecules, 2021.
• Krzyscik M, Sokolowska-Wedzina A, Jendryczko K, Pozniak M, Nawrocka D, Porebska N, Zakrzewska M, Otlewski J, Szlachcic A, Opaliński Ł, Preparation of site-specific cytotoxic protein conjugates via maleimide-thiol chemistryand sortase A-mediated ligation, Journal of Visualized Experiments, 2021.
• Porębska N, Poźniak M, Matynia A, Żukowska D, Zakrzewska M, Otlewski J, Opaliński Ł Galectins as modulators of receptor tyrosine kinases signaling in health and disease, Cytokines and Growth Factor Reviews, 2021.
• Szymczyk J, Sluzalska KD, Materla I, Opaliński Ł, Otlewski J, Zakrzewska M, FGF/FGFR-dependent molecular mechanisms underlying anti-cancer drug resistance, Cancers, 2021.
• Piatek K, Kutner A, Cacsire Castillo-Tong D, Manhardt T, Kupper N, Nowak U, Chodyński M, Marcinkowska E, Kallay E, Schepelmann M, Vitamin D Analogs Regulate the Vitamin D System and Cell Viability in Ovarian Cancer Cells, International Journal of Molecular Sciences, 2021.
• Gaikwad S, Gonzalez CM, Vilarino D, Lasanta G, Villaverde C, Mourino A, Verlinden L, Verstuyf A, Peluso-IItis C, Rochel N, Berkowska K, Marcinkowska E, Litocholic acid-based design of noncalcemic vitamin D receptor agonists, Bioorganic Chemistry, 2021.

2020

• Poźniak M, Sokołowska-Wędzina A, Jastrzębski K, Szymczyk J, Porębska N, Krzyścik MA, Zakrzewska M, Miączyńska M, Otlewski J, Opaliński Ł, FGFR1 clustering with engineered tetravalent antibody improves the efficiency and modifies the mechanism of receptor internalization, Molecular Oncology, 2020.
• Sochacka M, Opaliński Ł, Szymczyk J, Zimoch M, Czyrek A, Krowarsch D, Otlewski J and Zakrzewska M, FHF1 is a bona fide fibroblast growth factor that activates cellular signaling in FGFR-dependent manner, Cell Communication and Signaling, 2020.
• Doan KN, Grevel A, Martensson CU, Ellenrieder L, Thornton N, Wenz LS, Opaliński Ł, Guiard B, Pfanner N, Becker T, The Mitochondrial Import Complex MIM Functions as Main Translocase for alpha-Helical Outer Membrane Protein, Cell Reports, 2020.
• Jendryczko K, Chudzian J, Skinder N, Opaliński Ł, Rzeszótko J, Wiedlocha A, Otlewski J, Szlachcic A, FGF2-Derived PeptibodyF2-MMAE Conjugate for Targeted Delivery of Cytotoxic Drugs into Cancer Cells Overexpressing FGFR1, Cancers, 2020.
• Nawrocka D, Krzyscik MA, Opaliński Ł, Zakrzewska M, Otlewski J, Stable fibroblast growth factor 2 dimers with high pro-survival and mitogenic potential, International Journal of Molecular Sciences, 2020.
• Nowak U, Janik S, Marchwicka A, Łaszkiewicz A, Jakuszak A, Cebrat M, Marcinkowska E, Investigating the role of methylation in silencing of VDR gene expression in normal cells during hematopoiesis and in their leukemic counterparts, Cells, 2020.
• Fabisiak A, Brzeminski P, Berkowska K, Rarova L, Marcinkowska E, Sicinski RR, Design, synthesis and biological evaluation of novel 2-alkylidene 19-norcalcitriol analogs, Bioorganic Chemistry, 2020.
• Brzeminski P, Fabisiak A, Berkowska K, Rarova L, Marcinkowska E, Sicinski RR, Synthesis of Gemini analogs of 19-norcalcitriol and their platinum(II) complexes, Bioorganic Chemistry, 2020.