RESEARCH TOPICS

• Phenolic Lipids – occurrence, isolation, characterization, biological activities, biosynthesis
• Liposomes as drug carriers
• New amphiphiles for construction of supramolecular drug carriers
• Characterization of supramolecular drug carriers

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Equipment

• HPLC Waters – analytical (detectors – diode array, fluorescence, light scattering), ternary gradient, autosampler, Millennium software
• HPLC Waters – preparative (UV detector, fraction collector), Millennium software
• LCD HPLC preparative, isocratic and binary gradient
• Zetasizer 5000 (Malvern) for size distribution analysis (PCS) and zeta potential determination
• Liposomal extruders (from 1 ml to 900 ml)
• Spectrofluorimeter Kontron SFM-25
• Typhoon Phospho-Chemo-FluorImager (Molecular Dynamics)

Selected publications

1. A. Kozubek, J.H.P. Tyman, Resorcinolic Lipids, The Natural Non-isoprenoic Amphiphiles and Their Biological Activity. Chem. Rev. 99 (1999) 1-26
2. E. Przeworska, J. Gubernator, A. Kozubek Liposomal vesicles by resorcinolic lipids, single-chain phenolic amphiphiles from Anacardium occidentale L. Biochim. Biophys. Acta 1513 (2001) 75-81
3. M. Stasiuk, A. Jaromin, A. Kozubek The effect of merulinic acid on biomembranes Biochim Biophys Acta 1667 (2004) 215-221
4. A. Kozubek, J. H. P. Tyman Bioactive Phenolic Lipids in. Chemistry of Natural Compounds in Studies in Natural Products Chemistry vol 30 (Atta ur Rachman Ed.) 2005, 111-190
5. Gubernator J, Drulis-Kawa Z, Kozubek A. A simply and sensitive fluorometric method for determination of gentamicin in liposomal suspensions. Int J Pharm. 327 (2006) 104-109
6. Langner M., Kozubek A. Pharmacokinetic modulation with particulate drug formulations in: Mozafari M.R. (Ed.) Nanocarrier Technologies: Frontiers of Nanotherapy, Springer 2006, pp. 113-138
7. M. Siwko; S. Marrink;A. de Vries, A. Kozubek; A. .Schoot Uiterkamp;A. Mark. Does isoprene protect plant membranes from thermal shock? A molecular dynamics study. Biochim Biophys Acta, 1768 (2007) 198-206.
8. M. Stasiuk, D. Bartosiewicz, A. Kozubek.Inhibitory effect of some natural and semisynthetic lipids upon acetylcholinesterase activity. Food Chem. 108 (2007) 996-1001
9. M. Kulawinek, A. Jaromin, A. Kozubek, R. Zarnowski J. Alkylresorcinols in selected Polish rye and wheat cereals and whole-grain cereal products J Agric. Food Chem. 56 (2008) 7236-7242
10. M. Stasiuk, A. Kozubek Membrane perturbing properties of natural phenolic and resorcinoli lipids FEBS Lett. 582 (2008) 3607-3613