Mass Spectrometry Laboratory
The purchase of the LC-MS equipment was financially supported by the Research Equipment Fund (FAB) of the “Excellence Initiative — Research University” (IDUB) program for the University of Wroclaw.
Laboratory is oriented towards providing a complex scientific service related to protein mass spectrometry and proteomics for research projects carried out at the Faculty of Biotechnology. Scientific cooperation with outside parties is also possible.
Analysis inquiries and consultation please contact: michal.tracz2@uwr.edu.pl
Leader:
dr Michał Tracz 
Scientific advisor:
prof. dr hab. Artur Krężel
Equipment
Analysis scope
Protein analyses
- intact mass analysis of a protein or a peptide (RP-LC)
- pure protein modification or PTM analysis (CID or ETD fragmentation)
- targeted protein/peptide quantification from biological matrices (ToF-MRM)
- protein or peptide hydrogen-deuterium exchange structural analyses (HDX)
- native protein MS
Proteomics
- protein identification from biological matrices, PAGE gels (including SDS-PAGE) or after CoIP
- protein abundance estimation from biological matrices, PAGE gels (including SDS-PAGE) or after CoIP (Shot-gun proteomics, BioID etc.)
- protein PTM analyses from biological matrices (acetyl-proteomics, ubiquitinomics, phosphoproteomics etc.)
Non-protein analyses
- compound’s mass analysis + isotopic profile (RP-LC)
- compound’s MS/MS spectra analysis (CID fragmentation)
- targeted compound quantification from biological matrices (ToF-MRM)
Publications supported by the Laboratory
- Markowska, K., Litwin, I., Kończak, J., Misiorna, D., Broczkowska, A., Tomaszewska, P., Tracz, M., Haenen, M. & Kramarz, K. (2025). PolySUMOylation of PCNA and Rad52 restricts centromeric recombination in fission yeast. Nature Communications, https://doi.org/10.1038/s41467-025-65862-1
- Pomorski, A., Wu, S., Tracz, M., Orzeł, A., Bezdekova, J., Brambor, A., … Krężel, A. (2025). Biarsenical-based fluorescent labeling of metallothioneins as a method for ultrasensitive quantification of poly-Cys targets. Analytica Chimica Acta, https://doi.org/10.1016/j.aca.2024.343550
- Śmiga, M., Roszkiewicz, E., Ślęzak, P., Tracz, M., & Olczak, T. (2025). cAMP-independent Crp homolog adds to the multi-layer regulatory network in Porphyromonas gingivalis. Frontiers in Cellular and Infection Microbiology, https://doi.org/10.3389/fcimb.2025.1535009
- Mosna, K., Orzeł, A., Tracz, M., Wu, S., & Krężel, A. (2025). Modification of human metallothioneins by garlic organosulfur compounds, allicin and ajoene: direct effect on zinc homeostasis with relevance to immune regulation. BioMetals, https://doi.org/10.1007/s10534-025-00716-3
- Wolański, M., Płachetka, M., Naumouskaya, V., Strzałka, A., Tracz, M., Valietova, D., & Zakrzewska-Czerwińska, J. (2025). AdpA, a Global Regulator of Hundreds of Genes, Including Those for Secondary Metabolism, in Streptomyces venezuelae. Antibiotics, https://doi.org/10.3390/antibiotics14090878
- Hryniewicz-Jankowska, A., Tracz, M., Opiełka, I., Augoff, K., Czogalla, A., & Sikorski, A. F. (2025). Quantitative proteomic data of flotillin-2 interactome within detergent resistant membranes of HeLa cells. Data in Brief, https://doi.org/10.1016/j.dib.2025.111970.
- Peris-Díaz, M.D., Krężel, A. & Barran.P. (2025). Deciphering the safeguarding role of cysteine residues in p53 against H2O2-induced oxidation using high-resolution native mass spectrometry. Commun Chem. https://doi.org/10.1038/s42004-024-01395-w
- Ciura, K., Sobota, O., Chorążewska, A. et al. (2025). Reprogramming FGF1 from the natural growth factor to the engineered heparan sulphate biosensor. Cell Commun Signal, https://doi.org/10.1186/s12964-025-02269-x
- Porębska, N., Chorążewska, A., Ciura, K., Pomorski, A., Krężel, A., & Opaliński, Ł. (2025). Innately Fluorescent Tetravalent Cytotoxic Conjugate TetraFHER2-vcMMAE Engages Aggregation-Dependent Endocytosis of HER2 for Enhanced Intracellular Drug Delivery. Journal of Medicinal Chemistry, https://doi.org/10.1021/acs.jmedchem.5c00782.
- Duława-Kobeluszczyk, J., Strzałka, A., Tracz, M., Bartyńska, M., Pawlikiewicz, K., Łebkowski, T., … Others. (2024). The activity of CobB1 protein deacetylase contributes to nucleoid compaction in Streptomyces venezuelae spores by increasing HupS affinity for DNA. Nucleic Acids Research, https://doi.org/10.1093/nar/gkae418
- Kalka, M., Chorążewska, A., Gędaj, A., Żukowska, D., Ciura, K., Biaduń, M., … Opaliński, Ł. (2024). Engineered intrinsically fluorescent galectin-8 variants with altered valency, ligand recognition and biological activity. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2024.134371
- Peris-Díaz, M. D., Orzeł, A., Wu, S., Mosna, K., Barran, P. E., & Krężel, A. (2024). Combining Native Mass Spectrometry and Proteomics to Differentiate and Map the Metalloform Landscape in Metallothioneins. Journal of Proteome Research. https://doi.org/10.1021/acs.jproteome.4c00271
- Śmiga, M., Ślęzak, P., Tracz, M., Cierpisz, P., Wagner, M., & Olczak, T. (2024). Defining the role of Hmu and Hus systems in Porphyromonas gingivalis heme and iron homeostasis and virulence. Scientific Reports, https://doi.org/10.1038/s41598-024-82326-6
